P-35: Celecoxiband Silymarin Ameliorated The Varicocele-Induced Inflammation and Oxidative Stress; Evidence for CoxII,iNOS and Enzymatic Antioxidant Status

Background: Varicocele (VCL) exerts its impact via enhancing inflammation and down-regulating testicular endocrine and antioxidant statuses. Celecoxib (CCB) is a non-steroidal anti-inflammatory drug (NSAID), which its effects based on the inhibition of cyclooxygenase (COX) enzymes. On the hand, silymarin (SMN) is known for its remarkable anti-oxidative impact. Therefore, present study was designed in order to evaluate the protective effects of CCB and SMN on VCL-induced biochemical derangements in testicular tissue. Materials and Methods: VCL was induced in 24 mature male rats and then animals were randomly divided into four groups including; non-treated VCL-induced, CCB-treated (10 mg/kg, orally), SMN-treated (100 mg/kg, orally), SMN+CCB-treated groups. Following 60 days after VCL induction, the mRNA levels of CoxII and iNOS were assessed by using semiquantitative real time-PCR. Moreover, histological alterations and germinal cells RNA damage were analyzed by using light and epifluorescent microscopy, respectively. Finally, the tissue levels of glutathione peroxidase (GSH-px), superoxide dismutase (SOD) were evaluated. Results: CCB and SMN- especially in simultaneous form of administration- significantly (p<0.05) reduced VCL-induced tubular atrophy, connective tissue edema, germinal epithelium dissociation as well as RNA damage. RT-PCR results showed that CCB and CCB+SMNtreated groups showed significant (p<0.05) reduction in mRNA levels of COXII and iNOS versus non-treated VCL-induced group. Meanwhile, animals in SMN alone and SMN+CCB-treated groups showed remarkable (p<0.05) enhanced levels of GSH-px and SOD. Conclusion: Our data suggest that, CCB and SMN via two different mechanisms diminished VCL-induced damages. Accordingly, CCB by down-regulating the inflammation and SMN by up-regulating the enzymatic line of antioxidant capacity protected RNA contents of germinal cells. Ultimately, CCB and SMN are suggested as appropriate compounds for medical applications in VCL.